ABSTARCT

ABSTRACT

Background: Cryptostegia madagascariensis which is an endemic plant of Madagascar is use to manage wounds but few studies are dedicated to this species. Objectives: This investigation aims to assess phytochemical and pharmacological properties of this plant. Methods: Cryptostegia madagascariensis leaves collected on July 2022 from the Atsimo-andrefana region (Madagascar) were air-dried and powdered. Hydroalcoholic extract were prepared by maceration from the dry leaves powder. Preliminary phytochemical screening was carried out. Gel named EA which includes 10 mg/mL of hydroalcoholic extract was prepared. The biological potentials of EA and the hydroalcoholic extract were assessed trough an excision wound model and microdilution method, respectively. Results: The experimental conditions resulted in a yield of 8.98% for the hydroalcoholic extract which contains alkaloids, steroids, flavonoids, leuco-anthocyanins, tannins and polyphenols. External application of EA accelerates wound contraction and the differences between the diameter of the control and EA treated wounds are statistically significant since the third day of treatment (𝑃 < 0.05, n = 3). The hydroalcoholic extract was active against Proteus mirabilis ATCC 35659 (MIC = 2 mg/ml), Listeria monocytogenes ATCC 19114TM (MIC = 5 mg/ml), Bacillus cereus LMG6910 (MIC = 10 mg/ml), Bacillus megaterium ATCC8145 (MIC = 10 mg/ml) and Enterobacter cloacae ATCC13047 (MIC = 10 mg/ml). Conclusions: This study allowed to demonstrate both antibacterial and wound-healing potentials of Cryptostegia madagascariensis leaves extract, deducing that its use as antibacterial and wound-healing agents will be probable. Thus, it enriches scientific data concerning the investigated plant.
Keywords: Cryptostegia madagascariensis, wound-healing, antibacterial.

Authors Contact

| ANDRIANASOLO Rondro Keila ^1* | ANDRIANANTENAINA Rigobert ² | LAHADSON Stanislas Fulgence ³ |

ANDRIANERATSIZAFY Raotoarinony ¹ | ANDRIAMAMONJISOA Dimbiniala ⁴ |ANDRIAMBELOSON Herivony Onja ²

| end | RASOLOMAMPIANINA Rado ² |

Affiliation.

^1. Institute of Technology of Ambositra | Engineering School of Agronomy and Environnement | BP 306 | Madagascar |

^2.National Center for Environmental Research (CNRE) | Environmental Microbiology Laboratory | Antananarivo | Madagascar |

^3. University of Antananarivo | Natural Substances and Organic-Biological Chemistry Laboratory (LCSN/COB)| Antananarivo |

Madagascar |

^4. National Center for Application of Pharmaceutical Research (CNARP) | Antananarivo | Madagascar |

This article is made freely available as part of this journal's Open Access:

[ Doi : https://doi.org/10.5281/zenodo.15281590 ]

Keila-Ref7-4-20ajiras150425

PHYTOCHEMICAL STUDY, WOUND HEALING AND ANTIBACTERIAL POTENTIALS OF

Cryptostegia madagascariensis Bojer ex Decne (APOCYNACEAE) LEAVES

| ANDRIANASOLO Rondro Keila ^1* | ANDRIANANTENAINA Rigobert ² | LAHADSON Stanislas Fulgence ³ | ANDRIANERATSIZAFY

innov. res. appl. sci. 2025; 20(4):81-87.