Introduction: Madagascar has about 14,000 plant species, 80% of which are endemic and that it has an original flora by its biodiversity. The valorisation of these species through scientific research is necessary for its development. Indeed, in order to scientifically enhance the medicinal plants endemic to Madagascar, chemical and biological studies have been undertaken. Objective: The object of this study is to identify the active molecules of the acetate extract by NMR and to evaluate the antimicrobial and antioxidant potential of extracts of Garcinia chapelieri H. Perr. Materials and methods: The antioxidant and antimicrobial activities of the extracts (hexanic, ethyl acetate) and the chemical constituents of the most active extract of the bark were examined. Column chromatography and TLC methods allow the isolation of products. The structures of the isolated products were identified by concerted analysis of the 1D and 2D NMR spectra and by comparison with the literatures. Results: The results of antimicrobial activity showed the ineffectiveness of the extracts against most of the strains tested. In the quantitative test, the AcOEt extract is rich in antioxidant products than the hexanic extract. Garcinia chapelieri AcOEt extract ensure trapping of the DPPH 73.69 %.The value is around 1638.48 mM / mg / l of extract as equivalent to α-tocopherol with the concentration of 0.25 mg / ml. The fractionation of the ethyl acetate extract having a strong antioxidant activity led to the isolation of 4 known products ursolic acid (1) and the three flavonoids, including 5-hydroxy-4',7-dimethoxy-6,8-dimethylflavone (eucalyptin) (2) and the two biflavonoids morelloflavone (3) and 7 '' - O-β-D-glycosylmorelloflavone (4).
Keywords: Garcinia chapelieri H. Perr., triterpene, flavonoids, antifungal, antioxidant.

Authors Contact

^*Correspondant author and authors Copyright © 2021:

| Julio Hervé Andriamadio ^1*| Volasoa Herilalaina Victorine Rambeloson ²| Anne Wadouachi ³| Amélie Raharisololalao ⁴| and | Léa Herilala Rasoanaivo ⁴|

Affiliation.

^1. University of Antsiranana | Department of Chemistry, Faculty of Science | BP: 0, Antsiranana 201 | Madagascar |
^2. University of Fianarantsoa | Department of Chemistry | Fianarantsoa 301 | Madagascar |
^3. University of Picardie | Laboratory of Antimicrobial Glycochemistry and Agroressources FRE CNRS 3517 | Amiens | France |
^4. University of Antananarivo | Laboratoire de Chimie des Substances Naturelles et Chimie Organique Biologique (LCSN/COB ) | Antananarivo 101 | Madagascar |
^4. University of Antananarivo | Laboratoire de Chimie des Substances Naturelles et Chimie Organique Biologique (LCSN/COB) | Antananarivo 101 | Madagascar |

This article is made freely available as part of this journal's Open Access: ID | Andriamadio–Ref4-ajira070521 |

BIFLAVONOIDS ISOLATED FROM THE STEM BARK OF Garcinia chapelieri H. Perr. Clusiaceae

| Julio Hervé Andriamadio ^1*| Volasoa Herilalaina Victorine Rambeloson ²| Anne Wadouachi ³|
Amélie Raharisololalao ⁴| and | Léa Herilala Rasoanaivo ⁴|. Am. J. innov. res. appl. sci. 2021; 12(5):182-187.

| PDF FULL TEXT | | XML FILE | | Received | May 01, 2021 | | Accepted | May 10, 2021 | | Published | May 14, 2021 |