This article is made freely available as part of this journal's Open Access: ID|M’boh–Ref3-ajira221120|
Background: The cryopreservation for long period of mold strains of medical interest essential for research is complex. Our objective is to evaluate the impact of conservation with 10% of Glycerol and Brain Heart Infusion (BHI Broth) on the survival of molds of medical interest at -20°C. Methodology: This is an analytical study of the cultural and morphological characteristics of 1310 mold strains isolated from environmental samples (air, surface and dust) collected from all azimuths and preserved in 10% Glycerol and BHI Broth at -20°C for one year. Results: Among the 1310 isolated strains and revived, colony growth was observed in 1059 strains (80.84%) against 251 (19.16%) which showed no signs of development and 119 strains (9.08%) were contaminated by other mold species or yeasts. Fungal viable strains observed after cryopreservation are: Aspergillus fumigatus, Aspergillus flavus, Aspergillus versicolor, Aspergillus niger, Aspergillus terreus, Aspergillus ochraceus, Aspergillus clavatus, Aspergillus sp, Penicillium sp, Fusarium solani, Fusarium sp, Curvularia sp, Mucor sp, Rhizomucor sp, and Trichoderma sp.Conclusion: The ability to resist freezing in suspension medium 10% Glycerol and BHI Broth varied from strain to strain, but with a better cryopreservation for Aspergillus, Penicillium, Fusarium, Mucor, Rhizomucor and Trichoderma. Keywords: Characterization, Mould, Environnement, Health, cryopreservation, Glycerol, BHI Broth
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American Journal of innovative Research & Applied Sciences
