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| Info-AJIRAS-® Journal ISSN 2429-5396 (Online) / Reference CIF/15/0289M |
American Journal of Innovative Research & Applied Sciences
|
American Journal of innovative
Research & Applied Sciences
Research & Applied Sciences
ISSN 2429-5396 (Online)
OCLC Number: 920041286
OCLC Number: 920041286
| ISSN: 2429-5396 (e) | https://www.american-jiras.com | |
| Web Site Form: v 0.1.05 | JF 22 Cours, Wellington le Clairval, Lillebonne | France |
| Web Site Form: v 0.1.05 | JF 22 Cours, Wellington le Clairval, Lillebonne | France |
Abstarct7-December-2023
| DECEMBER | VOLUME 22 | ISSUE N° 6 | 2025 |
| ARTICLES | Am. J. innov. res. appl. sci. Volume 22, Issue - 6 (Pages 39-46 (December, 2025)
| ARTICLES | Am. J. innov. res. appl. sci. Volume 22, Issue - 6 (Pages 39-46 (December, 2025)
RESUME
Background: Natural products derived from medicinal plants remain indispensable sources of novel bioactive compounds. Objectives: This study comprehensively investigated the phytochemical profile, antioxidant potential, and acute toxicity of leaf extracts from Cryptostegia madagascariensis Bojer Ex Decne (Apocynaceae). Methods: Leaf extracts were prepared according to different methods. Fractions and isolated compound were obtained from total alkaloid extract using chromatographic techniques. Spectroscopic methods (NMR) were used for structure elucidation. Acute toxicity and DPPH radical scavenging assays were performed. Results: The extraction of the dry leaves powder yielded hydroalcoholic crude extract, total alkaloid extract, and six distinct extracts. The fractionation of the total alkaloid extract led to the isolation of Canthin-6-one. Evaluation of antioxidant activity across multiple extracts revealed significant radical-scavenging capacity. The methanol extract exhibited the highest potency in DPPH (IC50 = 1.23 mg/mL) assays, suggesting its potential as a natural antioxidant source. This activity may be partly attributed to phenolic compounds and synergistic interactions with other compounds. However, acute oral toxicity testing of the crude hydroalcoholic extract in animal models demonstrated dose-dependent toxicity, with a median lethal dose LD₅₀= 78.125 mg/kg. Conclusions. This marks the first report of antioxidant potential, acute toxicity, and the phytochemical profile of leaf extracts from Cryptostegia madagascariensis specie.
Keywords: Cryptostegia madagascariensis, phytochemical profile, antioxidant, acute toxicity, Apocynaceae.
Background: Natural products derived from medicinal plants remain indispensable sources of novel bioactive compounds. Objectives: This study comprehensively investigated the phytochemical profile, antioxidant potential, and acute toxicity of leaf extracts from Cryptostegia madagascariensis Bojer Ex Decne (Apocynaceae). Methods: Leaf extracts were prepared according to different methods. Fractions and isolated compound were obtained from total alkaloid extract using chromatographic techniques. Spectroscopic methods (NMR) were used for structure elucidation. Acute toxicity and DPPH radical scavenging assays were performed. Results: The extraction of the dry leaves powder yielded hydroalcoholic crude extract, total alkaloid extract, and six distinct extracts. The fractionation of the total alkaloid extract led to the isolation of Canthin-6-one. Evaluation of antioxidant activity across multiple extracts revealed significant radical-scavenging capacity. The methanol extract exhibited the highest potency in DPPH (IC50 = 1.23 mg/mL) assays, suggesting its potential as a natural antioxidant source. This activity may be partly attributed to phenolic compounds and synergistic interactions with other compounds. However, acute oral toxicity testing of the crude hydroalcoholic extract in animal models demonstrated dose-dependent toxicity, with a median lethal dose LD₅₀= 78.125 mg/kg. Conclusions. This marks the first report of antioxidant potential, acute toxicity, and the phytochemical profile of leaf extracts from Cryptostegia madagascariensis specie.
Keywords: Cryptostegia madagascariensis, phytochemical profile, antioxidant, acute toxicity, Apocynaceae.
Authors Contact
*Correspondant author and authors Copyright © 2025:
| ANDRIANASOLO Rondro Keila 1* | ANDRIANANTENAINA Rigobert 2 | LAHADSON Stanislas Fulgence 3 | PAGNA MAWOUMA Issah Julio 4 | ANDRIANERATSIZAFY Raotoarinony 1 | ANDRIAMAMONJISOA Dimbiniala 5 |ANDRIAMBELOSON Herivony Onja 2 | end | RASOLOMAMPIANINA Rado 2 |
Affiliation:
1. Institute of Technology of Ambositra | Engineering School of Agronomy and Environnement | BP 306 | Madagascar |
2. National Center for Environmental Research (CNRE) | Environmental Microbiology Laboratory | Antananarivo | Madagascar |
3. University of Antananarivo | Natural Substances and Organic-Biological Chemistry Laboratory (LCSN/COB) | Antananarivo | Madagascar |
4. University of Yaoundé | Faculty of Sciences | Department of Organic Chemistries | BP 812 Yaoundé | Cameroon |
5. National Center for Application of Pharmaceutical Research (CNARP) | Antananarivo | Madagascar |
This article is made freely available as part of this journal's Open Access:
[ Doi : https://doi.org/10.5281/zenodo.17968485 ] ID: [ Keila-Ref5-6-22ajiras171225 ]
*Correspondant author and authors Copyright © 2025:
| ANDRIANASOLO Rondro Keila 1* | ANDRIANANTENAINA Rigobert 2 | LAHADSON Stanislas Fulgence 3 | PAGNA MAWOUMA Issah Julio 4 | ANDRIANERATSIZAFY Raotoarinony 1 | ANDRIAMAMONJISOA Dimbiniala 5 |ANDRIAMBELOSON Herivony Onja 2 | end | RASOLOMAMPIANINA Rado 2 |
Affiliation:
1. Institute of Technology of Ambositra | Engineering School of Agronomy and Environnement | BP 306 | Madagascar |
2. National Center for Environmental Research (CNRE) | Environmental Microbiology Laboratory | Antananarivo | Madagascar |
3. University of Antananarivo | Natural Substances and Organic-Biological Chemistry Laboratory (LCSN/COB) | Antananarivo | Madagascar |
4. University of Yaoundé | Faculty of Sciences | Department of Organic Chemistries | BP 812 Yaoundé | Cameroon |
5. National Center for Application of Pharmaceutical Research (CNARP) | Antananarivo | Madagascar |
This article is made freely available as part of this journal's Open Access:
[ Doi : https://doi.org/10.5281/zenodo.17968485 ] ID: [ Keila-Ref5-6-22ajiras171225 ]
ARTICLE 4 PDF
Friday, September 19, 2025
PHYTOCHEMICAL STUDY, ACUTE TOXICITY AND DPPH RADICAL SCAVENGING ACTIVITY OF EXTRACTS FROM Cryptostegia madagascariensis BOJER EX DECNE (APOCYNACEAE) LEAVES Send Comments
| ANDRIANASOLO Rondro Keila 1* | ANDRIANANTENAINA Rigobert 2 | LAHADSON Stanislas Fulgence 3 | PAGNA MAWOUMA Issah Julio 4 | ANDRIANERATSIZAFY Raotoarinony 1 | ANDRIAMAMONJISOA Dimbiniala 5 |ANDRIAMBELOSON Herivony Onja 2 | end | RASOLOMAMPIANINA Rado 2 |. Am. J. innov. res. appl. sci. 2025; 22(6):39-46.
| PDF FULL TEXT | | XML FILE | | [ Doi: Keila-Ref5-6-22ajiras171225 ] |
| PDF FULL TEXT | | XML FILE | | [ Doi: Keila-Ref5-6-22ajiras171225 ] |